翻訳と辞書 Words near each other ・ Single Integrated Operational Plan ・ Single Intelligence Account ・ Single Island ・ Single Ladies ・ Single Ladies (Put a Ring on It) ・ Single Ladies (TV series) ・ Single Life ・ Single Life (film) ・ Single Lock Records ・ Single malt Scotch ・ Single malt whisky ・ Single market ・ Single Me Out (song) ・ Single molecule electric motor ・ Single molecule fluorescent sequencing ・ Single molecule real time sequencing ・ Single Mother ・ Single Mothers (album) ・ Single Mothers (band) ・ Single National Park ・ Single net lease ・ Single Non-Emergency Number ・ Single non-transferable vote ・ Single occupancy ・ Single of the Weak ・ Single of the Year ・ Single operator two radios ・ Single parent ・ Single particle analysis ・ Single particle tracking Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Single molecule real time sequencing ： ウィキペディア英語版 Single molecule real time sequencing Single molecule real time sequencing (SMRT) is a parallelized single molecule DNA sequencing method. Single molecule real time sequencing utilizes a zero-mode waveguide (ZMW). A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single nucleotide of DNA being incorporated by DNA polymerase. Each of the four DNA bases is attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses out of the observation area of the ZMW where its fluorescence is no longer observable. A detector detects the fluorescent signal of the nucleotide incorporation, and the base call is made according to the corresponding fluorescence of the dye. == Technology == The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level. The signal from a phospho-linked nucleotide incorporated by the DNA polymerase is detected as the DNA synthesis proceeds which results in the DNA sequencing in real time. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Single molecule real time sequencing」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.